ABSTRACT
Objective To study the refining of Radix Sophorae Flavescentis (RSF) decoction by microfiltration combined with ultrafiltration method,and to compare the refining effect with that refined by alcohol sedimentation method.Methods HPLC was applied to analyze the content of oxymatrine,ultroviolet (UV) spectrophotometry was used to determine the content of total flavonoids in RSF decoction,and suitable concentration of NaOH solution for the detergent were chosen in order to wash the membrane.Results The refining effect is better and the recovery ratio of efficient components is higher in RSF decoction refined by microfiltration combined with ultrafiltration method than in those refined by alcohol sedimentation method.Microfiltration combined with ultrafiltration method is of simple operation and short period,and ultrafiltration membranes can be easily washed.Conclusion The microfiltration combined with ultrafiltration method can be used as the alternative for the traditional alcohol sedimentation method in refining of RSF decoction.
ABSTRACT
Object To study the adsorption function of macroporous resin AB 8 for the purification of total flavones in Sophora flavescens Ait and the effects of concentration, pH, flow rate of the adsorbate and eluant Methods UV spectrophotometry was used to analyze the content of total flavone in S flavescens Results The appropriate adsorption conditions were: concentration of Sophora extract, 0 285 mg/mL ; pH=4; flow rate 3 BV/h, when 50% alcohol was used as eluant, the effect of desorption was satisfactory Conclusion AB 8 resin can be used to refine the total flavone in S flavescens
ABSTRACT
Objective: To study the refinement of general flavone of sophora flavescens decoction by macroporous resins combined with ceramic microfiltration membranes (MR-CM), and compare it with macroporous resin combined with alcohol-sedimentation (MR-AS). Methods: UV-spectrophotometry method was applied to analyze the content of general flavone of sophora flavescens. Results: The porous ratio and effect of eliminating impurity of general flavone of sophora flavescens decoction by MR-CM method was higher than those by MR-AS method, but the MR-CM method is of simple operation and short period.Conclusion: MR-CM method is better than alcohol-sedimentation-macroporous resin method in refining of general flavone of sophora flavescens decoction.